19-22 October 2011
Hotel Roanoke, Roanoke VA
US/Eastern timezone
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Microfluidic device for three-dimensional electrokinetic manipulation of single fluorescent molecules

20 Oct 2011, 14:18
Crystal Ballroom C (Hotel Roanoke, Roanoke VA)

Crystal Ballroom C

Hotel Roanoke, Roanoke VA


Jason King (University of Tennessee Space Institute)


The ability to manipulate and trap single molecules in solution through the application of actively controlled electric fields is a valuable tool for a number of bio-molecular studies of proteins and nucleic acids. Here we report the development of a microfluidic device consisting of four electrodes sputtered onto two glass coverslips and fixed in a tetrahedral arrangement. This geometrical configuration allows for a uniform electric field of any orientation through the application of appropriate voltages. Three-axis control has been demonstrated for micron-sized polystyrene beads and 40 nm fluorescent spheres in phosphate buffered solution. Previous work has characterized planar motion. Recent changes to the experimental setup include the addition of a cylindrical lens in the detection arm to quantify axial position and a National Instruments PCI-7833R to provide precise voltage control. Finally, a real-time tracking algorithm and its use for trapping will be discussed.


Brian Canfield (University of Tennessee Space Institute) Lloyd Davis (University of Tennessee Space Institute) William Hofmeister (University of Tennessee Space Institute)

Presentation Materials

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