19-22 October 2011
Hotel Roanoke, Roanoke VA
US/Eastern timezone
<font color=red><b>To upload your file, log in (@ top right), then click on your talk title, then click "Add Material" at the bottom of the page.</b></font>

Microfluidic device for three-dimensional electrokinetic manipulation of single fluorescent molecules

20 Oct 2011, 14:18
12m
Crystal Ballroom C (Hotel Roanoke, Roanoke VA)

Crystal Ballroom C

Hotel Roanoke, Roanoke VA

Speaker

Jason King (University of Tennessee Space Institute)

Description

The ability to manipulate and trap single molecules in solution through the application of actively controlled electric fields is a valuable tool for a number of bio-molecular studies of proteins and nucleic acids. Here we report the development of a microfluidic device consisting of four electrodes sputtered onto two glass coverslips and fixed in a tetrahedral arrangement. This geometrical configuration allows for a uniform electric field of any orientation through the application of appropriate voltages. Three-axis control has been demonstrated for micron-sized polystyrene beads and 40 nm fluorescent spheres in phosphate buffered solution. Previous work has characterized planar motion. Recent changes to the experimental setup include the addition of a cylindrical lens in the detection arm to quantify axial position and a National Instruments PCI-7833R to provide precise voltage control. Finally, a real-time tracking algorithm and its use for trapping will be discussed.

Co-authors

Brian Canfield (University of Tennessee Space Institute) Lloyd Davis (University of Tennessee Space Institute) William Hofmeister (University of Tennessee Space Institute)

Presentation Materials

Your browser is out of date!

Update your browser to view this website correctly. Update my browser now

×